Traditional laser scanning focal microscope contrast digital holographic microscope

Conventional laser scanning confocal microscope contrast digital holographic microscope DHM

Confocal laser scanning microscope (CLSM) uses laser as the scanning light source to scan the image point by point, line by line and face by side. The system scans the images of different planes through focusing and can be displayed by computer analysis and simulation. The stereostructure of the cell sample. The principle of scanning to achieve 3D is no different from traditional probe profilometers.

The principle of digital holographic microscope to realize 3D microscopy is to use CCD to record the hologram formed by object light and interference light. The hologram of the sample contains phase information and amplitude information. The amplitude information provides a traditional microscope contrast image, the phase information provides accurate 3D topographical information of the sample, and real-time 3D microscopy is achieved through computer reconstruction.

The digital holographic microscope DHM is characterized by its non-scanning imaging mode to achieve 3D topography microscopy, which can achieve ultra-high-speed real-time 3D microscopy. This is not possible with traditional 3D measurement systems such as CLSM, scanning probe microscope, atomic force microscope AFM, white light interferometer WLI.

Sample display: liquid lens deformation, red blood cell analysis, MEMS microactuator

Digital Holographic Microscope (DHM)

Principle: The hologram formed by the interference of CCD recorded object light and reference light is used to perform real-time numerical reconstruction according to the phase information and amplitude information contained in the hologram, and the accurate three-dimensional shape of the sample is obtained.

And the traditional optical microscope and electron microscope SEM, atomic force microscope AFM, laser scanning confocal microscope CLSM and other obvious advantages are its non-scanning imaging method, can achieve ultra-high-speed real-time dynamic 3D microscopy. The most current application of this feature is to characterize the dynamic response of MEMS devices in the field of MEMS MEMS. The laser Doppler vibrometer that is traditionally used in the MEMS field has an advantage in accuracy.

1. Liquid lens structure deformation

2. Graphene film is deformed by force

3. Heating volatiles of degradable materials

4, photopolymer deformation by light

The measurement principle is holography: the hologram formed by the interference between the CCD collected object light and the reference light contains the phase information of the object, and the 3D shape is reconstructed in real time.

Advantages: 1. Ultra-high-speed large-area 3D shape non-scanning real-time imaging, imaging rate can achieve 1000fps

2, the vertical can achieve sub-nanometer resolution

3, non-contact imaging, non-destructive samples, no fear of vibration

Typical user: Peking University Institute of Technology - Building a plane stress tympanic membrane test platform

Tsinghua University - Semiconductor

Huazhong University of Science and Technology -

Innovative 4D 3D Morphological Characterization——DHM for Dynamic 3D Morphological Characterization

Features:

1. Non-scanning, non-contact, ultra-high-speed large-area, dynamic three-dimensional shape representation (speed up to 1000fps)

2, vertical sub-nano resolution

3. Roughness measurement according to international standards, non-destructive samples without fear vibration

Principle: The CCD recording hologram contains the phase information of the object, and the real-time numerical reconstruction can obtain the real-time three-dimensional topographic map.

Application legend:

Graphene film deformation, roughness measurement, photopolymer deformation, liquid lens deformation, etc.

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