Institute of Yellow Sea: first identification of miRNA profile of semi-smooth tongue sole

The tongue slippery sole (Cynoglossus semilaevis) is one of the important commercial fishes in China. Currently, the cultivation of C. semilaevisis is threatened by serious bacteria and pathogens. Vibriosis is the most common bacterial infection in fish and can cause serious diseases in fish including C. semilaevisis, and Vibrio anguillarum is the main cause of the disease. As an important class of non-coding RNA, microRNA (miRNA) plays an important regulatory role in various biological processes including immune response. However, there is no research report on miRNAs participating in the immune response of bacteria in C. semilaevisis. To this end, the team led by Researcher Chen Songlin and Researcher Sha Zhenxia of the Yellow Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences used high-throughput sequencing technology for the first time to conduct a comprehensive analysis of the miRNA spectrum of C. semilaevisis under the infection of the bacteria In the May issue of Developmental and Comparative Immunology.

The researchers first constructed three small RNA libraries from the immune tissues (liver, head kidney, spleen, and intestine) of C. semilaevisis before and after V. anguillarum infection. CG), the other library is from the group infected with V. anguillarum and disease (referred to as HOSG), the third library is from the group infected with V. anguillarum and disease (referred to as NOSG), and then analyzed by high-throughput sequencing technology (The small RNA sequencing project is undertaken by Lianchuan Biology). The researchers obtained 11,216,875, 12,313,404, and 11,398,695 Clean Reads from the above three libraries. Bioinformatics analysis identified a total of 452 miRNAs, including 24 new miRNAs. Next, the researchers analyzed miRNAs that were differentially expressed between different libraries. In the NOSG-CG comparison, there were 175 miRNAs (38.72%) with significantly different expressions. In the HOSG-CG comparison, 215 miRNAs (47.57%) were significantly differentially expressed. Compared with CG, when comparing HOSG-NOSG, 122 miRNAs (26.99%) were found to be significantly differentially expressed. The selected 10 miRNAs were verified by real-time quantitative PCR, and the results were consistent with sequencing. Further analysis of the GO and KEGG pathways of the target genes of differentially expressed miRNAs showed that there is a group of highly abundant miRNAs that are significantly expressed under different conditions and may be involved in the development of the immune system and immune response.

This study reported for the first time the miRNA expression profile of C. semilaevisis in its immune tissues under V. anguillarum infection. The experiment found that many miRNAs showed significant differential expression under the infection of bacteria. This work also provides a basis for a deep understanding of the molecular mechanisms by which miRNA regulates C. semilaevisis host-bacteria interaction.

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