![<?echo $_SERVER['SERVER_NAME'];?>](/template/twentyseventeen/skin/images/header.jpg)
This reagent is for research use only: serum or plasma test principle: ox-LDL kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Standards with known ox-LDL concentration, samples with unknown concentration are added to microwells The detection is carried out in the microplate. First incubate ox-LDL and biotin-labeled antibody at the same time. After washing, HRP labeled with avidin was added. After incubation and washing, the unbound enzyme conjugate is removed, and then substrates A and B are added to act simultaneously with the enzyme conjugate. Produce colors. The color depth is proportional to the concentration of ox-LDL in the sample. Safety Avoid direct contact with the stop solution and substrates A and B. Once exposed to these liquids, rinse with water as soon as possible. Do not eat, drink, smoke or use cosmetics during the experiment. Do not use your mouth to absorb any ingredients in the kit. Handling precautions Reagents should be stored according to label instructions, and returned to room temperature before use. The sparse standards should be discarded and cannot be stored. The slats not used in the experiment should be immediately returned to the packaging bag, sealed and stored to avoid deterioration. Unused other reagents should be packed or covered. Do not mix reagents of different batches. Use before warranty. Use a disposable pipette tip to avoid cross-contamination, and avoid using a sampler with a metal part when drawing the stop solution and substrates A and B. Use a clean plastic container to prepare the washing solution. Mix all components and samples in the kit thoroughly before use. When washing the enzyme-labeled plate, it should be fully patted dry. Substrate A should evaporate to avoid opening the lid for a long time. Substrate B is sensitive to light and avoid prolonged exposure to light. Avoid contact with hands, it is toxic. The OD value should be read immediately after the experiment is completed. The order of adding reagents should be the same to ensure that all wells are incubated for the same time. Perform the incubation operation according to the time, the amount and order of the liquid indicated in the instructions. Sample collection, processing and storage methods Serum-avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins. After collecting blood, centrifuge at 1000 × g for 10 minutes to separate the serum and red blood cells quickly and carefully. Plasma ----- EDTA, citrate, heparin plasma can be used for detection. Centrifuge at 1000 × g for 30 minutes to remove particles. The cell supernatant was centrifuged at 1000 × g for 10 minutes to remove particles and polymers. Tissue homogenate ----- Mash the tissue with appropriate amount of saline. Centrifuge at 1000 × g for 10 minutes, and take the supernatant for storage. If the sample is not used immediately, it should be divided into small parts and stored at -70 ℃ to avoid repeated freezing. If possible, do not use hemolysis or hyperlipidemia. If there are a large number of particles in the serum, centrifuge or filter before testing. Do not thaw at 37 ° C or higher. Thaw at room temperature and ensure that the sample is thawed evenly and adequately. Before use, mix all reagents thoroughly. Don't make the liquid generate a lot of foam, so as to avoid adding a large number of bubbles during sample addition, which will cause errors in sample addition. The number of slats required is determined by the number of samples to be tested plus the number of standard products. Each standard and empty transfer chemotactic growth factor β1 well is recommended to be re-welled. Each sample is determined according to its own quantity, and those that can use multiple holes can be used as much as possible. The specimen was diluted 1: 1 with the specimen dilution solution and then added 50ul to the reaction well. Add 50ul of the diluted standard to the reaction well and 50ul of the sample to be tested in the reaction well. Immediately add 50ul of biotin-labeled antibody. Cover the membrane plate, gently shake and mix, and incubate at 37 ° C for 1 hour. Shake off the liquid in the wells, fill each well with washing liquid, shake for 30 seconds, shake off the washing liquid, pat dry with absorbent paper. Repeat this operation 3 times. If washing with a plate washer, the number of washes is increased once. Add 80 ul of streptavidin-HRP to each well, gently shake and mix, and incubate at 37 ° C for 30 minutes. Shake off the liquid in the wells, fill each well with washing liquid, shake for 30 seconds, shake off the washing liquid, pat dry with absorbent paper. Repeat this operation 3 times. If washing with a plate washer, the number of washes is increased once. Add 50ul of substrate A and B to each well, mix gently by shaking, and incubate at 37 ° C for 10 minutes. Avoid light. Remove the enzyme labeling plate and quickly add 50ul of stop solution. After adding the stop solution, the results should be measured immediately. The OD value of each well was measured at a wavelength of 450 nm. The results of standard No. 6 and above are non-linear, and accurate results cannot be obtained based on this standard curve. Kit performance 1. Sensitivity: The minimum detection concentration is less than No. 1 standard. Linearity of dilution. The correlation coefficient between the linear regression of the sample and the expected concentration is 0.990. 2. Specificity: Does not react with other cytokines. 3. Repeatability: The coefficients of variation within and between plates are less than 10%. Judgment and analysis of results 1. Instrument value: read the OD value of each well on a microplate reader with a wavelength of 450 nm. 2. Take the absorbance OD value as the ordinate (Y), and the corresponding ox-LDL standard concentration as the abscissa (X ), The corresponding curve is made, and the ox-LDL content of the sample can be converted from the standard curve to the corresponding concentration according to its OD value. 3. Detection value range: 0-400mmol / L4, sensitivity: 1.0 mmol / LP2P-R / RBBP6 (proliferation potential protein related) Proliferation potential related protein antibody 0.2mlP14ARF / p16-INK4a tumor suppressor gene p14 antibody 0.1mlP14ARF / p16- INK4a tumor suppressor gene p14 antibody 0.2mlP16 / CDKN2A (Cyclin-dependent kinase inhibitor-2A) tumor suppressor gene p16 antibody 0.1mlP16 / CDKN2A (Cyclin-dependent kinase inhibitor-2A) tumor suppressor gene p16 antibody 0.2mlP19ARF p19ARF tumor suppressor gene antibody 0.1 mlP19ARF p19ARF tumor suppressor gene antibody 0.2ml Mouse anti-goat IgG / PE fluorescein PE labeled mouse anti-goat IgG 0.1ml Mouse anti-goat IgM / PE fluorescein PE labeled mouse anti-goat IgM 0.1ml Mouse anti-human IgG / PE fluorescein Mouse labeled anti-human IgG 0.1ml Mouse anti-rabbit IgG / PE Fluorescein PE labeled fluorescein PE labeled mouse anti-human IgG Mouse anti-rabbit IgG 0.1ml Mouse anti-rat IgG / PE? Fluorescein PE labeled mouse anti-large Mouse IgG 0.1ml P21 / WAF1 / CIP1 p21 antibody 0.1ml P21 / WAF1 / CIP1 p21 antibody 0.2ml P27 / kip1 (cyclin-dependent kinase inhibitor 1B) P27 antibody / cyclin-dependent kinase inhibitor 0.1ml P27 / kip1 (cyclin-dependent kinase inhibi tor 1B) P27 antibody / cyclin dependent kinase inhibitor 0.2ml P2Y1 receptor / P2ry1 P2Y1 receptor antibody 0.2ml P2Y4 receptor / P2ry4 P2Y4 receptor antibody 0.2ml P311 protein nerve regeneration-related protein antibody 0.2ml P38 ERK / MAPK (MAPK14) mitogen activation Protein kinase p38 antibody 0.1ml Rabbit anti-pig IgG / Cy3 fluorescein Cy3 labeled rabbit anti-pig IgG 0.1ml Rabbit anti-Pig IgM / Cy3 fluorescein Cy3 labeled rabbit anti-pig IgM 0.1ml
Dermal Fillers plump up the skin and raise cheeks and jawline, which creates an appearance of a youthful face. That means your results can look more natural than if you used a treatment that only targets individual superficial wrinkles.
Women and men have embraced dermal fillers as an effective treatment for restoring volume, smoothing skin, and improving facial contours.
Recovery time varies for each patient and for each type of filler injected. You can resume most activities right away, but it is generally recommended that you avoid intense physical activity for the first 24-48 hours to minimize swelling and bruising.
Most conditions can be alleviated with topical icing and massage and will improve within a matter of hours or just a few days.
- Standard: 500mg/vial or 360mg/vial or 240mg/vial or 150mg/vial.
- It needs to be mixed with sterile water for injection and lidocaine, the particle size is 3-10 microns, and only needs to be shaken for 10 minutes to use.
- It can be used for 30G sharp needles or 27G blunt needles.
- Less pain, better customer experience.
- Poly-l-lactic acid can stimulate the regeneration of collagen and reduce foreign body reaction.
- Reborn PLLA Dermal Filler can be used in the face, neck, hands, chest, and buttocks, especially around the eyes.
Email:info@rimlessindustry.com
WhatsApp:+86 18004455830
Dermal Filler For Cosmetic Treatment,Fillers For Under Eye Wrinkles,Filler Anti Wrinkle Serum,Hyaluronic Acid Gel For Skin
Rimless Industry Co.,Ltd. , https://www.rebornplla.com