Non-invasive detection of changes in Cl-flow in the microenvironment of fetal lung epithelial cells

The transport of body fluids in the lung epithelial cells is governed by the Na + and Cl- flow regulation generated by the osmotic pressure on both sides of the epithelial cell membrane, and the energy required for the ion transport process is provided by the active Na + / K + ATP enzyme. Membrane distribution is controlled by ligand-adjusted ion channels and exchangers, so if the transport of Cl- is defective, it will lead to diseases related to dysregulation of body fluid transport.

Cystic fibrosis is caused by the lack of Cl-transport. For the study of this disease, the previously used detection methods, such as isotope tracing, did not detect the single Cl-transport in and out of the cell, but the overall situation of all ion transport into and out of the cell, so the conclusion was drawn It can not accurately explain the changes of Cl- inside and outside the cell. Non-damage micro-measurement technology (SrE) overcomes this problem by selecting Cl-self-reference electrode to detect changes in extracellular Cl-flow.

In this study, the selective Cl-self-reference electrode was used to detect the change direction of Cl-flow and extracellular Cl under the action of agonists in the basal or apical end of fetal lung epithelial cells in a real-time, high-resolution, and non-invasive manner. -Quantity changes. The results show that there is a close relationship between fluid transport and Cl-gradient in the physiological microenvironment of the distal apical membrane of the lung, and the kinetic gradient of Cl- can be used as a true indicator of Cl- in the plasma membrane microenvironment. This is very important for understanding the relationship between the concentration of salts in the fluid on the surface of the airway and the regulation of cellular and molecular processes and epithelial fluid transport.

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