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Pollination of flowering plants is a complex development process. The entire process is subject to strict genetic and cellular control, and extracellular signals play a key regulatory role. These signals include Ca2 +, pH, actin cytoskeleton, cAMP and GTPase, etc., "Science" prospects ion dynamics as a new research method to explain the regulation process in pollen tube growth. Ca2 +, K +, H + (pH) and Cl- affect the growth of pollen tubes and guide the growth direction. The interaction of extracellular signals and intracellular substances determines the direction of pollen tube growth. As a fine living structure, an organism can properly select ions and molecules to carry out a very smart engineering of ions and molecules. The measurement method of non-injury, non-contact or semi-injury under physiological conditions is very conducive to truly reveal the role of extracellular signals. At present, the non-damage micro-measurement technology can be used to dynamically measure the ion flow of pollen tubes or other samples without damage and contact, which provides the most direct means for exploring the effect of extracellular signals on organisms. Several articles have been published in "Plant Cell", "Plant Physiology" and other internationally renowned magazines. Keywords: ion fluxes, pollen tube, Ca2 +, H +, K +, Cl- References: KR Robinson, MA Messerli. Science's STKE (2002) 162: 1-3
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