How to prevent failure of liquid pump during operation

To maintain the good performance of the liquid chromatography pump, the system must be kept clean and the quality of the solvents and reagents used must be maintained. The mobile phase must be filtered and degassed before entering the flow path.
Preventing pump failure requires continuous and uninterrupted efforts:
1) The use of high-quality reagents and HPLC-grade solvents is good for pumps and chromatographic columns;
2) The mobile phase and solvent are filtered with high-quality materials before use;
3). Degas the mobile phase and solvent;
4), every time you start to use it, you should exhaust the gas and empty it. After the work is completed, wash the buffer or harmful substances from the pump and the chromatographic column; do not allow water and corrosive solvents and other harmful substances to stay in the pump and the chromatographic column;
5), regularly replace the online filter ring and other gaskets;
6), strictly follow the recommendations in the pump operation manual.
A pump in good operating condition should stabilize the baseline on the chromatogram; the repeatability of the retention time is good. The pressure fluctuation during isocratic elution is less than 2%. The pressure change during gradient elution should be slow and steady.
In order to eliminate the fault as soon as possible after the occurrence, the seal, check valve (inlet and outlet), pump head device, various joints, fuses and other parts should be always available, as well as replacement tools.

This article comes from how to prevent the failure of the liquid phase pump in the operation | Scientific Instrument Online Original Link: http: //

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