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High performance liquid chromatography has four important functions.
â‘ Separate and analyze the component content of the drug: Since a drug often contains multiple components, each vitamin B6, D1, A, E can be separated and the content can be determined at one time. Another example is analgesics or antipyretics. This method can also be used to separate and measure the content of each component.
â‘¡ Intermediate control in drug production: When producing antibiotics, we must first understand the existence of the main body and by-products in the fermentation broth. For example: the fermentation products of penicillin, tetracycline, cephalosporin, erythromycin, daunorubicin, gentamicin, etc., can be analyzed by C18 column and methanol and buffer mobile phase or by ion pair method to analyze the main body And the content of its products. For procaine amide, theophylline, etc., the main content can be determined, and for nitroglycerin, the main content and its degradation products can be determined to ensure the heat of the drug.
â‘¢ Liquid chromatograph analyzes the residual amount of drugs in the body: This is one of the main contents of the recent research on the efficacy of new drugs. For example, oridonin is a component in plants, which has anti-cancer effects, but is highly toxic. It is very important to determine its content in serum. Now you can use the C18 column and methanol water as the mobile phase to determine the serum content after treatment to obtain the blood content. If the content of sulfonamides in serum and urine is not allowed to be too large, C18 column and acidic ethanol can be used as the mobile phase. After determining the content, the most appropriate dosage can be estimated. Another example is tetracycline, barbituric acid, analgesics, antipyretics, diabetes drugs, etc., the content in blood or urine can be measured using C18 column with methanol and water as mobile phases. Therefore, the application of reversed-phase chromatography is extremely wide.
â‘£Determination of the metabolic products of drugs in various organs, especially to study the existence of metabolites of drugs in serum. Such as measuring keto acid and glutaric acid in serum. The measurement of metabolites in urine is more convenient and does not require complicated pretreatment. Because studying the components in urine can identify the metabolic function of patients, it has important reference value for pathological research or bed diagnosis.
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