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Ultra-high performance liquid chromatography is a brand new category in separation science. UPLC, with the help of HPLC theory and principles, covers new technologies such as small particle fillers, very low system volume, and rapid detection methods, increasing the throughput, sensitivity and Chromatographic peak capacity.
Ultra high performance liquid chromatography (UPLC) is an emerging field, as the world's first commercial UPLC product Waters
The ACQUITY UPLCTM ultra-high performance liquid chromatography system is also just emerging, so there is still a lack of published literature. Compared with traditional HPLC, the speed, sensitivity and resolution of UPLC are 9 times, 3 times and 1.7 times of HPLC respectively
Times. Therefore, it will be widely used in protein, peptide, metabolomics analysis and other biochemical fields. In addition,
In the analysis of natural products, using UPLC to connect with a mass spectrometer detector will greatly promote the development of natural product analysis, especially in the field of traditional Chinese medicine research.
When referring to "proteomics" or "metaboomics", the difference from the absence of "groups" is from the analytical point of view that the sample volume is extremely large, and thousands of samples need to be analyzed in a short time. The high speed advantage of UPLC without loss of resolution can be fully demonstrated here. Most biochemical samples and natural products are very complex. Figure 1 is a comparison of two chromatograms (ultraviolet detection) of HPLC and UPLC for peptide fingerprints. Under the same conditions, UPLC can separate chromatographic peaks by more than double that of HPLC. Figure 2 is a chromatogram of metabolite analysis. Under the same conditions, the resolution of UPLC can recognize more chromatographic peaks (mass spectrometer-
LCT).
Ultra high performance liquid chromatography (UPLC) is an emerging field, as the world's first commercial UPLC product Waters
The ACQUITY UPLCTM ultra-high performance liquid chromatography system is also just emerging, so there is still a lack of published literature. Compared with traditional HPLC, the speed, sensitivity and resolution of UPLC are 9 times, 3 times and 1.7 times of HPLC respectively
Times. Therefore, it will be widely used in protein, peptide, metabolomics analysis and other biochemical fields. In addition,
In the analysis of natural products, using UPLC to connect with a mass spectrometer detector will greatly promote the development of natural product analysis, especially in the field of traditional Chinese medicine research.
When referring to "proteomics" or "metaboomics", the difference from the absence of "groups" is from the analytical point of view that the sample volume is extremely large, and thousands of samples need to be analyzed in a short time. The high speed advantage of UPLC without loss of resolution can be fully demonstrated here. Most biochemical samples and natural products are very complex. Figure 1 is a comparison of two chromatograms (ultraviolet detection) of HPLC and UPLC for peptide fingerprints. Under the same conditions, UPLC can separate chromatographic peaks by more than double that of HPLC. Figure 2 is a chromatogram of metabolite analysis. Under the same conditions, the resolution of UPLC can recognize more chromatographic peaks (mass spectrometer-
LCT).
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